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Polymerase Chain Reaction (PCR) is a molecular
biology technique that allows for quick replication of
DNA. With PCR, minute quantities of genetic material can
be amplified millions of times within a few hours
allowing for the rapid and reliable detection of
infectious organisms, including the viruses that cause
AIDS and hepatitis, and other microorganisms that affect
women's health and cause tuberculosis.
PCR-based tests have several advantages over traditional
antibody-based diagnostic methods that measure the
body's immune response to a pathogen. In particular, PCR-based
tests are able to detect the presence of pathogenic
agents earlier than serologically-based methods, as
patients can take weeks to develop antibodies against an
infectious agent. Earlier detection of infection can
mean earlier treatment and an earlier return to good
health.
In SIRO Roche Light Cycler is used to quantify
the amount of virus in a person's blood ('viral load')
thereby allowing physicians to monitor their patient's
disease progression and response to therapy. Viral load
assessment before, during and after therapy has
tremendous potential for improving the clinical
management of diseases caused by viral infection,
including AIDS and hepatitis.
Real Time analysis offers
several advantages over the conventional technologies,
such as greater specificity, reproducibility and
sensitivity.
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